Biological IR and Raman Imaging
PhD School at the Faculty of SCIENCE at University of Copenhagen
This is a toolbox course where 80% of the seats are reserved for PhD students enrolled at the Faculty of SCIENCE at UCPH and 20% of the seats are reserved for PhD students from other Danish Universities/faculties (except CBS). Seats will be allocated on a first-come, first-served basis and according to the applicable rules.
Anyone can apply for the course, but if you are not a PhD student at a Danish university (except CBS), you will be placed on the waiting list until enrollment deadline. After the enrollment deadline, available seats will be allocated to applicants on the waiting list.
Aim and Content
This advanced imaging course module gives a hands-on introduction to chemical imaging of biological and environmental samples using IR and Raman micro-spectroscopy. In this way, the course aims to make students capable of integrating IR and/or RAMAN imaging in their projects. Depending on the needs of the enrolled students, the focus of the course can be broader than only exploiting environmental and/or biological samples.
The course gives an introduction to vibrational micro-spectroscopy, an overview of what can and cannot be detected by these techniques, and how they complement each other. In the course, we go through all steps of selecting analysis methods, sampling, and preparing specimens, carrying out measurements, processing data, and presenting results. Especially, we will discuss representability issues for heterogeneous samples, the spatial and spectral resolutions obtainable, and the compromises necessary when utilising micro-spectroscopy within research. Instrument demonstrations and hands-on training will be included in the course.
Learning outcomes
Intended learning outcome for the students who complete the course:
Knowledge:
• Able to give an overview of the molecular basis of vibrational spectroscopy
• Describe properties and characteristics of infrared radiation
• Describe how molecular vibrations can be detected and quantified by interaction with light
Skills:
• Read, understand and evaluate scientific papers that utilize IR and/or RAMAN imaging within the student’s own field
• Design a sampling and sample preparation methodology for vibrational microscopy based on known material properties and known characteristics and limitations of the included methods
• Make a flow-chart of data management from raw IR/Raman image data to analysis and presentation of results
Competences:
• Able to describe the difference between IR and RAMAN imaging
• Judge suitability of these imaging modalities to solve scientific questions within the student’s own field of research
• Design a procedure based on vibrational microscopy aimed at addressing a research question relevant to the student’s own field of research
Target Group
All students planning to use IR and/or Raman imaging in their projects.
Recommended Academic Qualifications
Open for master and PhD students after taking the UCPH’s basic Biological Imaging module (Biological Imaging – Basic Module” (Professor Alexander Schulz, PLEN)) or if the relevant knowledge was acquired in another way, e.g., through related courses at UCPH or other universities. If in doubt, please contact lgt@ign.ku.dk.
Research Area
Relevant for students within Environmental science, Material science, Biology, Biology-Biotechnology, Medicine and Technology, Molecular Biomedicine, Nanoscience or Pharmaceutical Sciences.
Teaching and Learning Methods
Lectures, demonstrations, hands-on practical exercises, theoretical exercises.
Students will prepare samples, perform measurements, analyse data and present their work in a written report.
Type of Assessment
Written report. The report must be evaluated as “passed” for the course to be completed.
Course coordinator
Lisbeth G. Thygesen
Guest Lecturers
TBD
Dates
3 last weeks of block 4.
Expected frequency
To be held every 2nd year
Course location
Frederiksberg Campus
Course fee
• Participant fee: 1000 DKK
• PhD student enrolled at SCIENCE: 0 DKK
• PhD student from Danish PhD school Open market: 0 DKK
• PhD student from Danish PhD school not Open market: 3000 DKK
• PhD student from foreign university: 3000 DKK
• Master's student from Danish university: 0 DKK
• Master's student from foreign university: 3000 DKK
• Non-PhD student employed at a university (e.g., postdocs): 3000 DKK
• Non-PhD student not employed at a university (e.g., from a private company): 8400 DKK
Cancellation policy
• Cancellations made up to two weeks before the course starts are free of charge.
• Cancellations made less than two weeks before the course starts will be charged a fee of DKK 3.000
• Participants with less than 80% attendance cannot pass the course and will be charged a fee of DKK 5.000
• No-show will result in a fee of DKK 5.000
• Participants who fail to hand in any mandatory exams or assignments cannot pass the course and will be charged a fee of DKK 5.000
Course fee and participant fee
PhD courses offered at the Faculty of SCIENCE have course fees corresponding to different participant types.
In addition to the course fee, there might also be a participant fee.
If the course has a participant fee, this will apply to all participants regardless of participant
type - and in addition to the course fee.