Digital PCR methods for circulating tumor DNA analyses - Theory and Practice
Doctoral School of Science and Environment at Roskilde University
The course is part theoretical and part practical with lectures on
• Introduction to dPCR,
• Optimization of ctDNA analyses,
• Sample QC strategies and controls,
• dPCR platform presentations, and
• dPCR result analysis
In the practical part of the course, students will work in small groups together with an instrument application specialist on the different dPCR platforms (Bio-Rad, Qiagen, Roche, Stilla and ThermoFisher invited). Plasma DNA samples, in-house ctDNA assays and vendor assays will be analyzed enabling a head-to-head comparison of the different dPCR platforms
In the practical part of the course, students will work in small groups together with an instrument application specialist on the different dPCR platforms (Bio-Rad, Stilla, Qiagen and Roche). Plasma DNA samples, in-house ctDNA assays and vendor assays will be analyzed enabling a head-to-head comparison of the different dPCR platforms.