Advanced flow cytometry II
Graduate School of Health and Medical Sciences at University of Copenhagen
This course is free of charge for PhD students at Danish universities (except Copenhagen Business School), and for PhD Students from NorDoc member faculties. All other participants must pay the course fee.
Anyone can apply for the course, but if you are not a PhD student at a Danish university, you will be placed on the waiting list until enrollment deadline. This also applies to PhD students from NorDoc member faculties. After the enrollment deadline, available seats will be allocated to applicants on the waiting list.
Learning objectives
A student who has met the objectives of the course will be able to:
1. Optimize staining of a sample and settings for the instrument before running samples
2. Design and make an instrument dependent spread matrix. Use the spread matrix for design of multicolor flow panels
3. Understand the standards of publishing and storing flow cytometry related data
4. Analyze and interpret data based on tSNE and FlowSOM software
5. Understand the principle of barcoding in flow cytometry
6. Design a multicolor experiment with relevant controls, fit for interpretation and publication
Content
The course is divided in 5 themes, each covering part of a flow cytometer experiment. We will take you through design of multi-color panels, how to get the best sensitivity on your measurements, what controls to pick for the experiment, and how to analyze/evaluate the experiment. We will also show you how barcoding of samples can optimize the time spend at the instruments and minimize variation of samples that have been taken at different time points e.g. kinetic studies in patients.
The course will include the use of FlowLogic and FlowJo for analyzing samples, as well as tSNE and FlowSOM for analysis and generation of hypothesis.
Participants
PhD students and people on similar level.
We recommend that you have had our Basic course in flow cytometry I or a similar course, and we recommend that you have worked with multicolor flow cytometry for at least a year, to get the most out of the course. There will be work on different instruments and software, and there will not be time to train people in basic use of an instrument and knowledge about flow cytometry.
Relevance to graduate programmes
The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH:
Immunology and Infectious Diseases
Language
English
Form
Each theme is a 2-week block where students have to watch some e-learning videos and read some papers to prepare for the practical part. In each theme, we will meet for 1 or 2 days to make practical and/or theoretical exercises, related to the theme.
Course director
Professor Jan Pravsgaard Christensen, ISIM, 35327873, jpc@sund.ku.dk
Teachers
Jan Pravsgaard Christensen, Professor, PhD, postdocs, and technicians at the department
Dates
24 Feb - 10 Mar, home studies to prepare for exercise, app 4-5 hours
11 Mar, 9-16, exercise
12-24 Mar, home studies to prepare for exercise, app 4-5 hours
25 Mar, 9-16, exercise
26 Mar, 9-16, exercise
27 Mar - 7 Apr, home studies to prepare for exercise, app 4-5 hours
8 Apr, 9-16, exercise
9-22 Apr, home studies to prepare for exercise, app 4-5 hours
23 Apr, 9-16, exercise
24 Apr, 9-16, exercise
25 Apr - 5 May, home studies to prepare for exercise, app 4-5 hours
6 May, 9-16, exercise
Course location
The Panum Institute, Blegdamsvej 3B, DK-2200 Copenhagen N. Maersk Tower, 14th floor.
Registration
Please register before 27/1-25
Expected frequency
We run the course in the spring (Feb. to Apr.) of each year
Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules.
Applications from other participants will be considered after the last day of enrolment.
Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.